{"id":4904,"date":"2025-10-20T15:55:37","date_gmt":"2025-10-20T06:55:37","guid":{"rendered":"https:\/\/jteccorp.wdkb.jp\/?page_id=4904"},"modified":"2026-05-29T15:47:27","modified_gmt":"2026-05-29T06:47:27","slug":"makcell","status":"publish","type":"page","link":"https:\/\/www.j-tec.co.jp\/english\/life-science\/makcell\/","title":{"rendered":"MakCell"},"content":{"rendered":"\n<div class=\"phead\">\n    <div class=\"c-life-title makcell en\">\n                <h1><span class=\"txt1\">Automated Cell Culture System MakCell<\/span> <span class=\"txt2\"><img src=\"\/assets\/img\/lifescience\/makcell\/logo.webp\" alt=\"MakCell\"><\/span><\/h1>\n                <p class=\"text-box\">Automated Medium Change<br>\nin a Cell-Friendly Environment<\/p>\n              <\/div>\n    <div class=\"c-localnavi\">\n        <ul class=\"localnavi-list\">\n            <li class=\"localnavi-item\">\n                <a href=\"#feature\">Product Features<\/a>\n            <\/li>\n            <li class=\"localnavi-item\">\n                <a href=\"#operation\">Operation Procedure<\/a>\n            <\/li>\n            <li class=\"localnavi-item\">\n                <a href=\"#application\">Application Notes<\/a>\n            <\/li>\n            <li class=\"localnavi-item\">\n                <a href=\"#spec\">Specifications \/<br class=\"sp\"> Related Items<\/a>\n            <\/li>\n        <\/ul>\n    <\/div>\n<\/div>\n<div class=\"l-detail-content__inner\">\n    <p class=\"c-lead \">MakCell performs medium change on your behalf !<\/p>\n<p class=\"c-summery\">MakCell is an automated cell culture system that performs medium exchange within a CO\u2082 incubator.\n  It enables cultivation of iPS cells and stem cells under an ISO Class 5-equivalent clean environment and reduces\n  contamination risk through a proprietary nozzle auto-cleaning function. It reduces manual workload and minimizes\n  physical stress on cells.<\/p>\n    <!-- MakCell Slide -->\n    <div class=\"makcellslide-wrap\">\n        <div class=\"c-makcellslider swiper swiper-initialized swiper-horizontal swiper-backface-hidden\">\n            <div class=\"swiper-wrapper\" id=\"swiper-wrapper-67a2c37d9a4388c4\" style=\"cursor: grab\">\n                <div class=\"swiper-slide swiper-slide-active\" role=\"group\" aria-label=\"1 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide1-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">Medium change schedules hinder other experiments. \n\t\t\t\t\t\tUnable to focus on core research. Tied down by weekend \n\t\t\t\t\t\tand after-hours medium change\u2026\n                    <\/p>\n                <\/div>\n                <div class=\"swiper-slide swiper-slide-next\" role=\"group\" aria-label=\"2 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide2-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">  Automate your medium change with MakCell.\n                    <\/p>\n                <\/div>\n                <div class=\"swiper-slide\" role=\"group\" aria-label=\"3 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide3-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">Reclaim your weekends and evenings by delegating\n\t\t\t\t\t\tafter-hours medium change.<\/p>\n                <\/div>\n                <div class=\"swiper-slide\" role=\"group\" aria-label=\"4 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide4-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">Focus on your research goals by delegating\n\t\t\t\t\t\tdaily medium change routines.<\/p>\n                <\/div>\n                <div class=\"swiper-slide\" role=\"group\" aria-label=\"5 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide5-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">Achieve high-quality research even with limited personnel\n\t\t\t\t\t\tby offloading routine tasks.<\/p>\n                <\/div>\n                <div class=\"swiper-slide\" role=\"group\" aria-label=\"6 \/ 6\" style=\"margin-right: 20px\">\n                    <div class=\"img\"><img src=\"\/assets\/img\/lifescience\/makcell\/slide6-en.svg\" alt=\"\">\n                    <\/div>\n                    <p class=\"makcell-slide-text\">Ensure continuous, stable cell \n\t\t\t\t\t\tculture and eliminate variability \n\t\t\t\t\t\tin results.<br>\n                        <a href=\"#feature\" class=\"c-button\">learn more<\/a><\/p>\n                <\/div>\n            <\/div>\n            <div class=\"swiper-button-next\" role=\"button\" aria-label=\"Next slide\">&nbsp;<\/div>\n            <div class=\"swiper-button-prev swiper-button-disabled\" role=\"button\" aria-label=\"Previous slide\">&nbsp;<\/div>\n        <span class=\"swiper-notification\"><\/span><\/div>\n    <\/div>\n\n    <!-- Product Features Section -->\n    <section class=\"c-section\">\n        <a id=\"feature\" class=\"u-scroll-point\"><\/a>\n        <h2 class=\"c-title l2\">Product Features<\/h2>\n        <div class=\"column -col3 u-mb40\">\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">Automated medium change in CO\u2082 incubator<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/feat01-2.webp\" alt=\"Automated medium change in CO\u2082 incubator\">\n                <\/div>\n                <div class=\"c-text\">\n                    <p class=\"u-mt10 u-fw-bold\">\u25cfReduces stress on cells caused by environmental changes such as CO\u2082 concentration, temperature, and humidity \u25cfReduces damage to cells caused by moving culture vessels (tremor) when changing culture medium (also applicable for dimple plates) <\/p>\n                <\/div>\n            <\/div>\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">Designed to minimize contamination<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/iso5graf-en.webp\" alt=\"Designed to minimize contamination\">\n                <\/div>\n                <div class=\"c-list u-mt10 c-text\">\n                    <p class=\"u-fw-bold\">\u25cf\u201dISO14644-1 Class 5\u2033 equivalent cleanliness environment due to circulation in the chamber with HEPA filter<\/p>\n                    <p class=\"u-fw-bold\">\u25cfCleanlines is kept by washing the waste liquid nozzles that come in contact with the medium after medium change<\/p>\n                <\/div>\n            <\/div>\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">Compatible with both dish and multi-well plates<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/haichi-en.svg\" class=\"u-w100\" alt=\"Compatible with both dish and multi-well plates\">\n                <\/div>\n                <div class=\"u-mt10 c-text\">\n                    <p class=\"u-fw-bold\">\u25cfCapable of operating simultaneously in different types of culture vessels<\/p>\n                    <ul class=\"c-list u-mt10\">\n                        <li>Dishes<br>\u03a660 mm \/ \u03a6100 mm<\/li>\n                        <li>Multi-well plates<br>6-well \/ 12-well \/ 24-well<\/li>\n                    <\/ul>\n                    <p class=\"u-mt10 u-fs14\">Settable culture vessels \u201c\u03a660 mm \/ \u03a6100 mm dish\u201d or \u201c6-well \/ 12-well \/ 24-well plate\u201d<\/p>\n                <\/div>\n            <\/div>\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">LCD touch panel on the front of the MakCell<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/touch-panel-en.webp\" alt=\"LCD touch panel on the front of the MakCell\">\n                <\/div>\n                <div class=\"u-mt10 c-text\">\n                    <p class=\"u-fw-bold\">\u25cfLCD display of temperature and CO\u2082 concentration in the incubator<\/p>\n                    <p class=\"u-fs14\">Manual operation of the MakCell is carried out using this panel. *Culture condition and schedule can be set up on the control PC.<\/p>\n                <\/div>\n            <\/div>\n            <div class=\"item wide\">\n                <h3 class=\"c-col2__title\">Three types of culture medium can be set separately (with culture medium cooling function)<\/h3>\n                <div class=\"c-col2 -half -gap20 -spcol2\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/feat02.webp\" alt=\"Three types of culture medium can be set separately\"><img src=\"\/assets\/img\/lifescience\/makcell\/30do.svg\" alt=\"30\u00b0 tilt\">\n                <\/div>\n                <div class=\"u-mt10 c-text\">\n                    <div class=\"makcell-itemflex\">\n                        <dl>\n                            <dt>\u25cfThree types of culture medium can be set separately, enabling the supply of culture medium tailored to the cells. A cooling unit is mounted on the top of the device to prevent the medium from deteriorating.<\/dt>\n                        <\/dl>\n                        <dl>\n                            <dt>\u25cfAbout the warning lamp for insufficient medium<\/dt>\n                            <dd class=\"u-mb10\"> Lamp illuminates when the cooling section is switched on.<br> Lamp flashes when the remaining volume of a 500 mL bottle is less than 100 mL or when the remaining volume of a 1000 mL bottle is less than 200 mL. <\/dd>\n                        <\/dl>\n                        <dl>\n                            <dt>\u25cfAbout the storage angle of the medium bottle<\/dt>\n                            <dd>The bottles are set at a 30-degree angle. This makes it easy to remove the bottle and to suck up the medium without leaving it to the end.<\/dd>\n                        <\/dl>\n                    <\/div>\n                <\/div>\n            <\/div>\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">Bottles for collecting discarded medium<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/feat04.webp\" alt=\"Bottles for collecting discarded medium\">\n                <\/div>\n                <div class=\"u-mt10 c-text\">\n                    <p class=\"u-fw-bold\">\u25cfHolds up to three wide-mouth medium bottles (1 liter) <\/p>\n                    <p> Storage in an easy-to-see location makes it easy to check discarded culture medium and prevents forgetting to replace bottles.<\/p>\n                <\/div>\n            <\/div>\n            <div class=\"item\">\n                <h3 class=\"c-col2__title\">Easy operation with control PC<\/h3>\n                <div class=\"c-image\">\n                    <img src=\"\/assets\/img\/lifescience\/makcell\/PC.webp\" alt=\"Easy operation with control PC\">\n                <\/div>\n                <div class=\"u-mt10 c-text\">\n                    <p class=\"u-fw-bold\">\u25cfCulture schedules can be managed on the control PC and up to 50 culture schedules can be registered.<\/p>\n                    <p class=\"note\"> Daily and weekly schedules can be checked at a glance.<\/p>\n                <\/div>\n            <\/div>\n        <\/div>\n        <div><\/div>\n    <\/section>\n    <div class=\"c-fix-button__buttons -inpage\">\n        <a href=\"\/english\/contact\/life-science-contact\/\" class=\"c-fix-button__btn -contact\">\n            <span>Contact<\/span>\n        <\/a>\n    <\/div>\n    <!-- Option Section -->\n    <section class=\"c-section\">\n        <a id=\"option\" class=\"u-scroll-point\"><\/a>\n        <h2 class=\"c-title l2\">Option<\/h2>\n        <div class=\"c-col3\">\n            <div class=\"\">\n                <h3 class=\"c-title l3\">eMail notification function<\/h3>\n                <div class=\"u-mt10 c-text\">\n                    <ul class=\"c-list u-mt20\">\n                        <li>Notification by eMail of the beginning and the end of medium change operations or the occurrence of errors<\/li>\n                        <li>The operating status can be checked even outside working hours, such as on holidays, for peace of mind. <\/li>\n                        <li>The notification items can be selected according to the settings.<\/li>\n                    <\/ul>\n                <\/div>\n            <\/div>\n            <div class=\"\">\n                <h3 class=\"c-title l3 \">Possible to change medium in a low-oxygen atmosphere<\/h3>\n                <ul class=\"c-list u-mt20\">\n                    <li> Cell culture can be carried out while maintaining a low-oxygen concentration environment. <p class=\"u-fs14 u-mt10\">Controlling the oxygen concentration contributes to optimal cell culture.<\/p>\n                    <\/li>\n                <\/ul>\n            <\/div>\n            <div class=\"\">\n                <h3 class=\"c-title l3 \">Clean booth<\/h3>\n                <ul class=\"c-list u-mt20\">\n                    <li>Installing the MakCell in a clean environment sufficiently lowers the risk of contamination. <p class=\"u-fs14 u-mt10\">Improved workability<\/p>\n                    <\/li>\n                <\/ul>\n            <\/div>\n        <\/div>\n    <\/section>\n    <!-- Operation Procedure Section -->\n    <section class=\"c-section\">\n        <a id=\"operation\" class=\"u-scroll-point\"><\/a>\n        <h2 class=\"c-title l2\">Operation Procedure<\/h2>\n        <div class=\"c-process\">\n            <ol class=\"c-process__steps\">\n                <li class=\"c-process__step is-silver\">\n                    <span class=\"c-process__number\">1<\/span>\n                    <p class=\"c-process__body\"> Set tubes and medium Nozzle cleaning recipe registration <\/p>\n                    <span class=\"c-process__arrow\"><\/span>\n                <\/li>\n                <li class=\"c-process__step is-lightblue\">\n                    <span class=\"c-process__number\">2<\/span>\n                    <p class=\"c-process__body\"> Register the type of culture vessel and the location (address) where it will be installed <\/p>\n                    <span class=\"c-process__arrow\"><\/span>\n                <\/li>\n                <li class=\"c-process__step is-blue\">\n                    <span class=\"c-process__number\">3<\/span>\n                    <p class=\"c-process__body\"> Register the amount and schedule of medium to be changed for each vessels\n                        Select nozzle cleaning recipe <\/p>\n                    <span class=\"c-process__arrow\"><\/span>\n                <\/li>\n                <li class=\"c-process__step is-navy\">\n                    <span class=\"c-process__number\">4<\/span>\n                    <p class=\"c-process__body\"> Automated change of medium on schedule <\/p>\n                <\/li>\n            <\/ol>\n        <\/div>\n        <div class=\"c-video col1 video-container\">\n            <iframe loading=\"lazy\" width=\"100%\" height=\"435\" src=\"https:\/\/www.youtube.com\/embed\/y-a93ofozrM\" frameborder=\"0\" allowfullscreen><\/iframe>\n        <\/div>\n    <\/section>\n    <div class=\"c-fix-button__buttons -inpage\">\n        <a href=\"\/english\/contact\/life-science-contact\/\" class=\"c-fix-button__btn -contact\">\n            <span>Contact<\/span>\n        <\/a>\n    <\/div>\n    <section class=\"c-section\">\n        <a id=\"application\" class=\"u-scroll-point\"><\/a>\n        <h2 class=\"c-title l2\">Application Notes<\/h2> <div class=\"c-applinote\">  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Culture of Intestinal Epithelial Cell Sheet Models with Cell Culture Inserts using the Tabletop Automated Cell Culture System &#8220;MakCell&#8221;<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2026\/01\/Cultivation-of-Intestinal-Epithelial-Cell-Sheet-Models-using-Cell-Culture-Inserts-with-MakCell_R-300x226.png\" alt=\"Culture of Intestinal Epithelial Cell Sheet Models with Cell Culture Inserts using the Tabletop Automated Cell Culture System &#8220;MakCell&#8221;\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2026\/01\/MakCell-Cell-Culture-Inserts-EN.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>Intestinal epithelial cell sheets are essential for drug testing, yet producing them is labor-intensive, requiring weeks of frequent and delicate medium changes. We automated this demanding process using MakCell. After 21 days, the sheets exhibited characteristic structures such as tight junctions and microvilli. With no intercellular leakage, the quality was comparable to manual culture. <\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Hypoxic culture of HeLa cells using MakCell<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/11\/HypoxiaHeLa-thumb_R-1-300x225-1.png\" alt=\"Hypoxic culture of HeLa cells using MakCell\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/09\/Hypoxic-Culture-of-HeLa-Cells-Using-MakCell_EN.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>We evaluated the cellular response under hypoxic conditions using MakCell. When HeLa cells were cultured in a MakCell incubator set to 1% O\u2082, HIF1\u03b1\u2014a well-known hypoxia response factor\u2014was confirmed to translocate into the nucleus within one hour. Similarly, nuclear translocation of HIF1\u03b1 was also confirmed in adipose-derived mesenchymal stromal cells.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Stability in hypoxic environments of MakCell<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/12\/Confirmation-of-stability-in-hypoxic-environments-using-MakCell-300x225-1.png\" alt=\"Stability in hypoxic environments of MakCell\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/03\/Stability-in-hypoxic-environments-of-MakCell.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>We evaluated the stability of MakCell in hypoxic environments. It took approximately 1 hour to go from normal oxygen concentration to hypoxic conditions with 5% O<sub>2<\/sub>. We then measured the fluctuations in hypoxia that occurred when the incubator door was opened and closed. After the door was opened for about 5 seconds, the hypoxia returned to the set oxygen concentration in about 6 minutes at the 5% O<sub>2<\/sub>&nbsp;setting and about 12 minutes at the 1% O<sub>2<\/sub>&nbsp;setting. Finally, it was confirmed that the hypoxic condition was maintained without disturbance during MakCell operation.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Cardiomyocyte differentiation from human iPS cells using MakCell<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/12\/MakCell-iPS-Cardiomyocyte-Differentiation-21-300x225-1-1.jpg\" alt=\"Cardiomyocyte differentiation from human iPS cells using MakCell\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/01\/Cardiomyocyte-differentiation-from-human-iPS-cells-using-MakCell.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>Differentiation of human iPS cells into cardiomyocytes was induced using GSK and Wnt inhibitors. The differentiation protocol was performed automatically using MakCell by replacing the media containing the inhibitors. The positive rate of cardiomyocyte marker cTnT at day 15 of differentiation was evaluated, and a positive rate of approximately 60% was obtained under the conditions of 2 days of GSK inhibitor followed by 2 days of Wnt inhibitor.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Early mesoderm differentiation using MakCell<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/12\/ApplicationData_MakCell_Early-Mesoderm-Differentiation_JP-300x225-1-1.png\" alt=\"Early mesoderm differentiation using MakCell\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2024\/03\/Early-endoderm-differentiation-using-MakCell.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>Early mesoderm differentiation of human iPS cells was induced by Automated medium change of three different media using MakCell. By programming the timing of the medium change, we were able to confirm gene expression of Brachyury and PDGFR\u03b1 by the addition of CHIR99021 as in manual differentiation induction.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Analysis of medium component using MakCell\u2019s culture<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/12\/Analysis-of-medium-component-using-MakCells-culture-300x225-1.png\" alt=\"Analysis of medium component using MakCell\u2019s culture\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2024\/03\/Analysis-of-medium-component-using-MakCells-culture.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>Lactate concentration, glucose concentration, and pH were analyzed before and after automated medium change using MakCell. MakCell was able to remove lactate and provide fresh medium as well as manual operation. There was no difference in the morphology or proliferation rate of iPS cells after 7 days of culture, and the undifferentiated nature of iPS cells was maintained after 2 months of medium change.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <div class=\"c-applinote__item\">\r\n        <h3 class=\"c-applinote__title\">Dimpled plate medium change by MakCell<\/h3>\r\n        <div class=\"flex\">\r\n            <div class=\"c-applinote__image\">  <img src=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2025\/12\/Dimpled-plate-medium-change-by-MakCell-300x225-2.jpg\" alt=\"Dimpled plate medium change by MakCell\">   <a href=\"https:\/\/www.j-tec.co.jp\/english\/wp-content\/uploads\/sites\/3\/2024\/03\/Dimpled-plate-medium-change-by-MakCell.pdf\" class=\"c-button -pdf\" target=\"_blank\" rel=\"noopener\">PDF<\/a>  <\/div>\r\n            <div class=\"c-applinote__content\">\r\n                <div class=\"c-applinote__text\"><!-- wp:paragraph -->\n<p>Using the MakCell for medium change during culture of cell aggregates in a dimple plate, the diameters of the cell aggregates were more uniform than when done manually. MakCell eliminates the need to carry the dimple plate from incubator to bench during medium change. In addition, the drop rate of the culture medium can be set to a constant and slow rate, making it possible to perform tasks that require careful attention during medium change automatically.<\/p>\n<!-- \/wp:paragraph --><\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>  <\/div>\n    <\/section>\n    <div class=\"c-fix-button__buttons -inpage\">\n        <a href=\"\/english\/contact\/life-science-contact\/\" class=\"c-fix-button__btn -contact\">\n            <span>Contact<\/span>\n        <\/a>\n    <\/div>\n    <section class=\"c-section\">\n        <a id=\"spec\" class=\"u-scroll-point\"><\/a>\n        <h2 class=\"c-title l2\">Specifications \/ Related Items<\/h2>\n        <h3 class=\"c-title l3 u-mt40 u-mb20\">Main Specifications<\/h3>\n        <div class=\"c-table blue -scroll\">\n            <table>\n                <tbody>\n                    <tr>\n                        <th colspan=\"2\">Model<\/th>\n                        <td>MC1000<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\" class=\"-w50\">Dimensions<\/th>\n                        <td>Width 746 mm \u00d7 Depth 682 mm \u00d7 Hight 1000 mm<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Weight<\/th>\n                        <td>100 kg<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Power Supply<\/th>\n                        <td>AC 100 V 15A<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Power Consumption (Device)<\/th>\n                        <td>800 W<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Leakage Circuit Breaker<\/th>\n                        <td>Rated Sensitivity Current: 30 mA<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Display<\/th>\n                        <td>6.5 inch LCD touch panel<\/td>\n                    <\/tr>\n                    <tr>\n                        <th rowspan=\"5\">Medium supply line system<\/th>\n                        <th>Pump type<\/th>\n                        <td>Peristaltic pumps: 3 units<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Culture Medium Refrigeration<\/th>\n                        <td>4\uff5e10\u2103<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Medium cooling element<\/th>\n                        <td>Peltier element<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Designated bottles<\/th>\n                        <td>Corning PET storage bottles 500 mL (model no. 431532) \/ 1000 mL (model no. 431533)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Recommended sterilisation method for nozzles<\/th>\n                        <td>Autoclave Sterilizable Stainless Steel Nozzle (MC-NZ)\u203bDetachable<\/td>\n                    <\/tr>\n                    <tr>\n                        <th rowspan=\"3\">Discard liquid line system<\/th>\n                        <th>Pump type<\/th>\n                        <td>Peristaltic pumps: 3 units<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Designated bottles<\/th>\n                        <td>General-purpose wide-mouth bottles (model no. GL-45)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Recommended sterilisation method for nozzles<\/th>\n                        <td>Autoclave Sterilizable Stainless Steel Nozzle (MC-NZ)\u203bDetachable<\/td>\n                    <\/tr>\n                    <tr>\n                        <th rowspan=\"14\">Incubator<\/th>\n                        <th>Internal Dimensions<\/th>\n                        <td>Width 680 mm \u00d7 Depth 550 mm \u00d7 Hight 375 mm<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Internal Volume<\/th>\n                        <td>140 L<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Cleanliness of the incubation chamber<\/th>\n                        <td>Equivalent to ISO14644-1 Class 5<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Maintaining a clean environment in the incubation chamber<\/th>\n                        <td>HEPA filter cleaning while circulating air in the incubation chamber<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Temperature indication<\/th>\n                        <td>Digital (Measurement variability of \u00b10.1 degrees)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Temperature Setting<\/th>\n                        <td>Touch Panel (0.1\u2103 Increments)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Temperature Setting Range<\/th>\n                        <td>20\uff5e40\u2103<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Temperature Uniformity<\/th>\n                        <td>\u00b10.5\u2103\uff08at 37\u2103 setting)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>CO<sub>2<\/sub> concentration indication<\/th>\n                        <td>Digital (Measurement variability of \u00b10.1%)<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>CO<sub>2<\/sub> Concentration Setting Range<\/th>\n                        <td>1.0\uff5e20.0\uff05 \uff080.1% Increments\uff09<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>CO<sub>2<\/sub> Gas Supply Pressure<\/th>\n                        <td>Approximately 0.1\u00b10.05 MPa<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Humidification method \/ Attained humidity<\/th>\n                        <td>Natural evaporation from water table \/ 90%RH or higher<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Specifications of turntable for culture vessel<\/th>\n                        <td> \u30fbSimultaneous upper and lower two-tier turntable<br>\u30fbMaximum number of culture vessels that can be set: 24 dishes or 12 multi-well plates<br>Note: If using 12 multi-well plates, the number of dishes is reduced to a maximum of 12. <\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Settable culture vessels<\/th>\n                        <td> \u30fbDishes\uff1a60\/100 \uff4d\uff4d<br>\u30fb Multi-well plates\uff1a 6\uff0f12\uff0f24<br>\u203bOnly SBS standard plates are compatible. <\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Ambient temperature<\/th>\n                        <td>20\uff5e24\u2103<\/td>\n                    <\/tr>\n                    <tr>\n                        <th colspan=\"2\">Ambient humidity<\/th>\n                        <td>30\uff5e70\uff05<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n\n  <p class=\"u-mt40\" style=\"font-weight: 700\">\u25a0MakCell is for research use only.<\/p>\n\n        <h2 class=\"c-title l2 u-mt40 u-mb20\">Culture medium tube set<\/h2>\n        <h3 class=\"c-title l3 u-mt20 u-mb10\">Tube set for 500 mL \/ 1000 mL bottle supply<\/h3>\n        <div class=\"c-table blue -scroll\">\n            <table>\n                <tbody>\n                    <tr>\n                        <th class=\"-w30\">Set Contents<\/th>\n                        <td>\u30fbElectron Beam Sterilized Single-Use Supply Tubes: 15 pcs<br>\u30fbCaps for unsterilised GL-45 medium supply bottles(Orange): 3 pcs*<br>*Please sterilize by autoclave, etc. before use.<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Model<\/th>\n                        <td>MC-DT-500 \/ MC-DT-1000<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Recommended replacement frequency<\/th>\n                        <td>At the end of each culture<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n        <h3 class=\"c-title l3 u-mt20 u-mb10\">Tube set for 500 mL \/ 1000 mL bottle supply with low adsorption of factors in the medium<\/h3>\n        <div class=\"c-table blue -scroll\">\n            <table>\n                <tbody>\n                    <tr>\n                        <th class=\"-w30\">Set Contents<\/th>\n                        <td>\u30fbElectron Beam Sterilized Single-Use Supply Tubes: 15 pcs<br>\u30fbCaps for unsterilised GL-45 medium supply bottles(Orange): 3 pcs*<br>*Please sterilize by autoclave, etc. before use.<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Model<\/th>\n                        <td>MC-DT-CF-500 \/ MC-DT-CF-1000<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Recommended replacement frequency<\/th>\n                        <td>At the end of each culture<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n        <h3 class=\"c-title l3 u-mt20 u-mb10\">Tube set for the discard medium<\/h3>\n        <div class=\"c-table blue -scroll\">\n            <table>\n                <tbody>\n                    <tr>\n                        <th class=\"-w30\">Set Contents<\/th>\n                        <td>\u30fbElectron Beam Sterilized Single-Use Supply Tubes: 15 pcs<br>\u30fbCaps for unsterilised GL-45 medium supply bottles(Blue): 3 pcs*<br>*Please sterilize by autoclave, etc. before use.<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Model<\/th>\n                        <td>MC-ST<\/td>\n                    <\/tr>\n                    <tr>\n                        <th>Recommended replacement frequency<\/th>\n                        <td>At the end of each culture<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n        <h3 class=\"c-title l3 u-mt40 u-mb20\">Other consumables<\/h3>\n        <div class=\"c-table blue -scroll\">\n            <table>\n                <tbody>\n                    <tr>\n                        <th class=\"-w50\">Air vent filter for culture medium supply bottle cap<\/th>\n                        <td> Sales unit: 15 pcs per unit<br> Model: MC-FL <\/td>\n                    <\/tr>\n                    <tr>\n                        <th>HEPA Filter for the incubation chamber<\/th>\n                        <td> Sales unit: by 1 unit<br> Model: MC-HF<br> Recommended replacement frequency: Once a year*<br>*Replacement at the time of periodic inspection (optional) <\/td>\n                    <\/tr>\n                    <tr>\n                        <th>MakCell Autoclave-Compatible Nozzle<\/th>\n                        <td> Sales unit: by 1 piece \/ by 6 pieces<br> Model: MC-NZ \/ MC-NZ-6 <\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n        <p class=\"u-mt40\" style=\"font-weight: 700\">\u25a0These Consumables are for research use only.<\/p>\n    <\/section>\n<\/div>\n\n\n\n<div class=\"c-fix-button\">\n    <button class=\"c-fix-button__close\" aria-label=\"Close\">\n      \n        <img loading=\"lazy\" src=\"\/assets\/img\/icon-close02.svg\" alt=\"\" width=\"24\" height=\"24\">\n      \n    <\/button>\n    <div class=\"c-fix-button__inner\">\n      <p class=\"c-fix-button__text\">\n        For product inquiries, click here\n      <\/p>\n      <div class=\"c-fix-button__buttons\">\n        <a href=\"\/english\/contact\/life-science-contact\/\" class=\"c-fix-button__btn -contact\">\n          <span>Contact<\/span>\n          \n            \n          \n        <\/a>\n      <\/div>\n    <\/div>\n  <\/div>\n","protected":false},"excerpt":{"rendered":"<p>Equipped with a built-in HEPA filter, the CO\u2082 incubator enables Automated medium change and supports the use of up to three different media types. It accommodates both culture dishes (100 mm \/ 60 mm) and multiwell plates (6-, 12-, and 24-well), making it suitable not only for maintenance culture but also for differentiation protocols.<\/p>\n","protected":false},"author":1,"featured_media":7313,"parent":4897,"menu_order":32,"comment_status":"closed","ping_status":"closed","template":"page-life-science-detail.php","meta":[],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/pages\/4904"}],"collection":[{"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/comments?post=4904"}],"version-history":[{"count":24,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/pages\/4904\/revisions"}],"predecessor-version":[{"id":7928,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/pages\/4904\/revisions\/7928"}],"up":[{"embeddable":true,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/pages\/4897"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/media\/7313"}],"wp:attachment":[{"href":"https:\/\/www.j-tec.co.jp\/english\/wp-json\/wp\/v2\/media?parent=4904"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}