- Life Science
Using CELLFLOAT technology to culture a large number of cells and large cell tissues
Product Features
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By utilizing our unique 3D rotational floating culture technology “CELLFLOAT”, we can efficiently culture a large number of cells and large cell tissues at once using large-capacity culture vessel.
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With the CellPet 3D-L, you can customize the shape and capacity of the culture vessels, as well as the gas exchange mechanism for the culture medium, according to your cultivation conditions. Additionally, it supports autoclave and EOG sterilization.
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The size of the CellPet 3D-L main body can be customized to fit the culture vessels, CO2 incubators, and installation space you are using.
We will flexibly accommodate your requests, so please feel free to consult us.
Culture Achievements
Example of large-scale culture of human osteosarcoma-derived cell line “MG63” using large-capacity culture vessel and CellPet 3D-L
Culture Medium: D-MEM (High Glucose) + 10% FBS + Antibiotics
Cultivation Period: 12 days
Cultivation Environment: Inside CO2 Incubator (Chamber Temperature: 37℃, CO2 Concentration: 5%)
－Other Cultivation Examples－
- ① Co-culture of human liver cancer-derived cells “HepG2”, Human umbilical vein endothelial cells “HUVEC”, and Human mesenchymal stem cells (MSC)
- ② Reconstruction of large elastic cartilage tissues using elastic cartilage progenitor cells derived from human auricle
Large-capacity vessel
Large-scale culture of the human osteosarcoma-derived cell line “MG63”
Examples of Customization
■Examples of Customization for Culture Vessels
Large-capacity culture vessel
(Capacity: 550 mL, with gas exchange mechanism)
Capable of cultivating a large number of cells and large cell tissues at once
Medium-capacity culture vessels
(Capacity: 180 mL each, series-connected, with gas exchange mechanism)
Capable of cultivating different types of cells and under different culture conditions simultaneously
■Examples of Customization for CellPet 3D-L
Optimization according to container size
When the capacity of the culture vessel is fixed by the customer, we will propose an optimized CellPet 3D-L that matches that size.
Addition of function for adjusting the floating position of cells (tissues) using images
It is possible to add a function for large tissue or mass cell cultures where a camera attached to the side of the culture vessel monitors the floating position of cells or tissues. By automatically adjusting the rotation speed of the vessel, the optimal floating position within the vessel can be maintained. This function enables a more stable cultivation environment.
Reference: Mechanism of gas exchange system in culture vessel
Inside the culture vessel equipped with a gas exchange mechanism, there is a cylinder wrapped in a gas-permeable membrane. Gases introduced from the outside (external air) dissolve into the culture medium through this permeable membrane. For instance, when used in a CO₂ environment, CO₂ dissolves into the culture medium according to the environmental partial pressure.