- Life Science
MakCell performs medium change on your behalf !
Product Features
Automatic medium change in CO₂ incubator
●Reduces stress on cells caused by environmental changes such as CO₂ concentration, temperature, and humidity ●Reduces damage to cells caused by moving culture vessels (tremor) when changing culture medium (also applicable for dimple plates)
Designed to minimize contamination
●”ISO14644-1 Class 5″ equivalent cleanliness environment due to circulation in the chamber with HEPA filter
●Cleanlines is kept by washing the waste liquid nozzles that come in contact with the medium after medium change
Compatible with both dish and multi-well plates
●Capable of operating simultaneously in different types of culture vessels
- Dishes
Φ60 mm / Φ100 mm - Multi-well plates
6-well / 12-well / 24-well
Settable culture vessels “Φ60 mm / Φ100 mm dish” or “6-well / 12-well / 24-well plate”
LCD touch panel on the front of the MakCell
●LCD display of temperature and CO₂ concentration in the incubator
Manual operation of the MakCell is carried out using this panel. *Culture condition and schedule can be set up on the control PC.
Three types of culture medium can be set separately (with culture medium cooling function)
- ●Three types of culture medium can be set separately, enabling the supply of culture medium tailored to the cells. A cooling unit is mounted on the top of the device to prevent the medium from deteriorating.
- ●About the warning lamp for insufficient medium
- Lamp illuminates when the cooling section is switched on.
Lamp flashes when the remaining volume of a 500 mL bottle is less than 100 mL or when the remaining volume of a 1000 mL bottle is less than 200 mL.
- ●About the storage angle of the medium bottle
- The bottles are set at a 30-degree angle. This makes it easy to remove the bottle and to suck up the medium without leaving it to the end.
Bottles for collecting discarded medium
●Holds up to three wide-mouth medium bottles (1 liter)
Storage in an easy-to-see location makes it easy to check discarded culture medium and prevents forgetting to replace bottles.
Easy operation with control PC
●Culture schedules can be managed on the control PC and up to 50 culture schedules can be registered.
Daily and weekly schedules can be checked at a glance.
Option
eMail notification function
- Notification by eMail of the beginning and the end of medium change operations or the occurrence of errors
- The operating status can be checked even outside working hours, such as on holidays, for peace of mind.
- The notification items can be selected according to the settings.
Possible to change medium in a low-oxygen atmosphere
- Cell culture can be carried out while maintaining a low-oxygen concentration environment.
Controlling the oxygen concentration contributes to optimal cell culture.
Clean booth
- Installing the MakCell in a clean environment sufficiently lowers the risk of contamination.
Improved workability
Operation Procedure
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1
Set tubes and medium Nozzle cleaning recipe registration
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2
Register the type of culture vessel and the location (address) where it will be installed
-
3
Register the amount and schedule of medium to be changed for each vessels Select nozzle cleaning recipe
-
4
Automatic change of medium on schedule
Application Notes
Hypoxic culture of HeLa cells using MakCell
We evaluated the cellular response under hypoxic conditions using MakCell. When HeLa cells were cultured in a MakCell incubator set to 1% O₂, HIF1α—a well-known hypoxia response factor—was confirmed to translocate into the nucleus within one hour. Similarly, nuclear translocation of HIF1α was also confirmed in adipose-derived mesenchymal stromal cells.
Stability in hypoxic environments of MakCell
We evaluated the stability of MakCell in hypoxic environments. It took approximately 1 hour to go from normal oxygen concentration to hypoxic conditions with 5% O2. We then measured the fluctuations in hypoxia that occurred when the incubator door was opened and closed. After the door was opened for about 5 seconds, the hypoxia returned to the set oxygen concentration in about 6 minutes at the 5% O2 setting and about 12 minutes at the 1% O2 setting. Finally, it was confirmed that the hypoxic condition was maintained without disturbance during MakCell operation.
Cardiomyocyte differentiation from human iPS cells using MakCell
Differentiation of human iPS cells into cardiomyocytes was induced using GSK and Wnt inhibitors. The differentiation protocol was performed automatically using MakCell by replacing the media containing the inhibitors. The positive rate of cardiomyocyte marker cTnT at day 15 of differentiation was evaluated, and a positive rate of approximately 60% was obtained under the conditions of 2 days of GSK inhibitor followed by 2 days of Wnt inhibitor.
Early endoderm differentiation using MakCell
Early mesoderm differentiation of human iPS cells was induced by automatic medium change of three different media using MakCell. By programming the timing of the medium change, we were able to confirm gene expression of Brachyury and PDGFRα by the addition of CHIR99021 as in manual differentiation induction.
Analysis of medium component using MakCell’s culture
Lactate concentration, glucose concentration, and pH were analyzed before and after automated medium change using MakCell. MakCell was able to remove lactate and provide fresh medium as well as manual operation. There was no difference in the morphology or proliferation rate of iPS cells after 7 days of culture, and the undifferentiated nature of iPS cells was maintained after 2 months of medium change.
Dimpled plate medium change by MakCell
Using the MakCell for medium change during culture of cell aggregates in a dimple plate, the diameters of the cell aggregates were more uniform than when done manually. MakCell eliminates the need to carry the dimple plate from incubator to bench during medium change. In addition, the drop rate of the culture medium can be set to a constant and slow rate, making it possible to perform tasks that require careful attention during medium change automatically.
Specifications / Related Items
Main Specifications
| Model | MC1000 | |
|---|---|---|
| Dimensions | Width 746 mm × Depth 682 mm × Hight 1000 mm | |
| Weight | 100 kg | |
| Power Supply | AC 100 V 15A | |
| Power Consumption (Device) | 800 W | |
| Leakage Circuit Breaker | Rated Sensitivity Current: 30 mA | |
| Display | 6.5 inch LCD touch panel | |
| Medium supply line system | Pump type | Peristaltic pumps: 3 units |
| Culture Medium Refrigeration | 4～10℃ | |
| Medium cooling element | Peltier element | |
| Designated bottles | Corning PET storage bottles 500 mL (model no. 431532) / 1000 mL (model no. 431533) | |
| Recommended sterilisation method for nozzles | Autoclave Sterilizable Stainless Steel Nozzle (MC-NZ)※Detachable | |
| Discard liquid line system | Pump type | Peristaltic pumps: 3 units |
| Designated bottles | General-purpose wide-mouth bottles (model no. GL-45) | |
| Recommended sterilisation method for nozzles | Autoclave Sterilizable Stainless Steel Nozzle (MC-NZ)※Detachable | |
| Incubator | Internal Dimensions | Width 680 mm × Depth 550 mm × Hight 375 mm |
| Internal Volume | 140 L | |
| Cleanliness of the incubation chamber | Equivalent to ISO14644-1 Class 5 | |
| Maintaining a clean environment in the incubation chamber | HEPA filter cleaning while circulating air in the incubation chamber | |
| Temperature indication | Digital (Measurement variability of ±0.1 degrees) | |
| Temperature Setting | Touch Panel (0.1℃ Increments) | |
| Temperature Setting Range | 20～40℃ | |
| Temperature Uniformity | ±0.5℃（at 37℃ setting) | |
| CO2 concentration indication | Digital (Measurement variability of ±0.1%) | |
| CO2 Concentration Setting Range | 1.0～20.0％ （0.1% Increments） | |
| CO2 Gas Supply Pressure | Approximately 0.1±0.05 MPa | |
| Humidification method / Attained humidity | Natural evaporation from water table / 90%RH or higher | |
| Specifications of turntable for culture vessel | ・Simultaneous upper and lower two-tier turntable ・Maximum number of culture vessels that can be set: 24 dishes or 12 multi-well plates Note: If using 12 multi-well plates, the number of dishes is reduced to a maximum of 12. |
|
| Settable culture vessels | ・Dishes：60/100 ｍｍ ・ Multi-well plates： 6／12／24 ※Only SBS standard plates are compatible. |
|
| Ambient temperature | 20～24℃ | |
| Ambient humidity | 30～70％ | |
Culture medium tube set
Tube set for 500 mL / 1000 mL bottle supply
| Set Contents | ・Electron Beam Sterilized Single-Use Supply Tubes: 15 pcs ・Caps for unsterilised GL-45 medium supply bottles(Orange): 3 pcs* *Please sterilize by autoclave, etc. before use. |
|---|---|
| Model | MC-DT-500 / MC-DT-1000 |
| Recommended replacement frequency | At the end of each culture |
Tube set for 500 mL / 1000 mL bottle supply with low adsorption of factors in the medium
| Set Contents | ・Electron Beam Sterilized Single-Use Supply Tubes: 15 pcs ・Caps for unsterilised GL-45 medium supply bottles(Orange): 3 pcs* *Please sterilize by autoclave, etc. before use. |
|---|---|
| Model | MC-DT-CF-500 / MC-DT-CF-1000 |
| Recommended replacement frequency | At the end of each culture |
Tube set for the discard medium
| Set Contents | ・Electron Beam Sterilized Single-Use Supply Tubes: 15 pcs ・Caps for unsterilised GL-45 medium supply bottles(Blue): 3 pcs* *Please sterilize by autoclave, etc. before use. |
|---|---|
| Model | MC-ST |
| Recommended replacement frequency | At the end of each culture |
Other consumables
| Air vent filter for culture medium supply bottle cap | Sales unit: 15 pcs per unit Model: MC-FL |
|---|---|
| HEPA Filter for the incubation chamber | Sales unit: by 1 unit Model: MC-HF Recommended replacement frequency: Once a year* *Replacement at the time of periodic inspection (optional) |
| MakCell Autoclave-Compatible Nozzle | Sales unit: by 1 piece / by 6 pieces Model: MC-NZ / MC-NZ-6 |